First Report of Cucurbit Chlorotic Yellows Virus Infecting Cucumber Plants in Spain

HomePlant DiseaseVol. 105, No. 8First Report of Cucurbit Chlorotic Yellows Virus Infecting Cucumber Plants in Spain PreviousNext DISEASE NOTE OPENOpen Access licenseFirst SpainR. Chynoweth, D. Jimenez, Liberti, Bellon-Dona, A. Carralero-González, Crespo-Sempere, and M. R. Albiach-MartiR. ChynowethBASF, Finca Lo Ruiz S/30593 La Palma, Cartagena, Murcia, SpainSearch for more papers by this author, JimenezBASF, LibertiBASF, Bellon-DonaBASF, Carralero-GonzálezPlant Pathology Microbiology Laboratory, ValGenetics, Scientific Park University Valencia, 46980 Paterna, Crespo-SemperePlant Albiach-Marti†Corresponding author: Albiach-Marti; E-mail Address: [email protected]://orcid.org/0000-0002-0413-495XPlant author AffiliationsAuthors Affiliations Chynoweth1 Jimenez1 Liberti1 Bellon-Dona1 Carralero-González2 Crespo-Sempere2 Albiach-Marti2 † 1BASF, 2Plant Published Online:22 Sep 2021https://doi.org/10.1094/PDIS-12-20-2553-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleDuring the winter 2018, symptoms leaf chlorotic spots followed interveinal chlorosis severe basal leaves were observed cucumber cultivar Laredo (Cucumis sativus) plants three independent greenhouses, sited distinct locations southern Spain. In all cases, Bemisia tabaci populations on infected plants. The symptomology was similar that caused whitefly-transmitted yellow stunting disorder virus (CYSDV, genus Crinivirus, family Closteroviridae), which is usually found infecting geographical area (Rubio et al. 2001). Samples from four different greenhouses collected tested presence CYSDV. Total RNA extracted samples using NucleoSpin Plant kit (Macherey-Nagel, Germany). Molecular detection CYSDV performed multiplex degenerate primer RT-PCR method (Wintermantel 2010), specific region highly conserved RNA-dependent polymerase (RdRp) gene criniviruses, also detects other criniviruses such as lettuce infectious yellows (LIYV) beet pseudo-yellows (BPYV). Results indicated viral species CYSDV, LIYV, BPYV not detected cucurbit plant samples. 2004, an emergent crinivirus (Cucurbit virus, CCYV), inducing those described cucurbits Japan (Okuda 2010). Recently, CCYV 2011 Greece (Orfanidou 2014) 2014 Egypt (Amer 2015) Saudi Arabia (Al-Saleh 2015). Therefore, a previously (Hamed 2011). Specific primers designed amplify 336 nt capsid protein (CP) 680 RdRp gene, located genomic 1 2, respectively. clear cDNA bands both expected sizes each sample, then purified sequenced via Sanger technology. BLAST analysis sequences showed 99% identity with nucleotide sequence CP genes isolates (LT992911, LT992910), China (KY400633.1, KX118632), Taiwan (JF502222). To our knowledge, first report It possible has spread throughout Mediterranean basin, remaining undetected due yellowing symptom similarities between CCYV. Detection represents new threat horticultural Europe.The author(s) declare no conflict interest.References:Al-Saleh, A., 2015. Dis. 99:734. https://doi.org/10.1094/PDIS-11-14-1101-PDN Link, ISI, Google ScholarAmer, Int. J. Virol. 11:1. https://doi.org/10.3923/ijv.2015.1.11 Crossref, ScholarHamed, K., 2011. 95:1321. https://doi.org/10.1094/PDIS-04-11-0349 ScholarOkuda, M., 2010. Phytopathology 100:560. https://doi.org/10.1094/PHYTO-100-6-0560 ScholarOrfanidou, C., 2014. 98:1446. https://doi.org/10.1094/PDIS-03-14-0311-PDN ScholarRubio, L., 2001. Gen. 82:929. ScholarWintermantel, W. Methods 170:106. https://doi.org/10.1016/j.jviromet.2010.09.008 ScholarThe interest.DetailsFiguresLiterature CitedRelated Vol. 8 August 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionComparison banana silicon-deficient soil amended or nonamended calcium silicate Fusarium oxysporum f. sp. cubense (W. Zellner al.). Photo credit: Fortunato F. Rodrigues. Infected spicebush tree outer bark showing black vascular discoloration sapwood typical laurel wilt (R. Olatinwo Olatinwo. Maize naturally Bipolaris zeicola (S. S. Liu Liu. Metrics Downloaded 479 times Article History Issue Date: Dec 2021Published: 22 2021First Look: 30 Jul 2021Accepted: 25 Feb 2021 Page: 2258 Information© American Phytopathological SocietyKeywordsCriniviruscucumberCCYVepidemiologydisease development spreadpathogen detectionwhitefliesvegetablesBemisia tabaciThe interest.